Given modern technology, environmental microbiology ...

Gram staining method was developed and introduced Hans Christian Gram and published in 1884. The method is based on the chemical composition of the cell wall of bacteria. Because of differences in chemical structure of the cell wall not less than 4 species of gram-stained organisms are defined by: Gram-positive, Gram-negative, gram-variable and gram-negative groups unknown. This technique is used as a tool to distinguish between two major groups of bacteria: Gram-positive and gram-negative in microbiology laboratories medical diagnosis of bacterial infections. Given modern technology, environmental microbiology used other methods, e. by PCR

tests DNA genetic markers to identify different bacteria. Gram is still used in medical microbiology, as a quick way to get a glimpse of the bacteria involved in infectious process. Its advantage is the speed of the result against 24 or more hours in anticipation of strattera online bacterial cultures. Thus, patients with suspected bacterial infection have body fluid or biopsy material colored by Gram, and microbiological culture. bacteria include proetobacteria, cyanobacteria, spirochetes, green sulfur and green

no sulfur bacteria. The group also contains relevant medical gram-

cocci, bacilli and many bacteria associated with nosocomial infections. These organisms stain pink and are a source of endotoxin. Bacillus, Staphylococcus, Streptococcus, Enterococcus,

dyplokokk pneumonia, Clostridium. These organisms stain purple. Mostly a result of bacteria in a mixed form of color: pink and purple, because their cell walls, are particularly sensitive to breakage, making and gram-negative and gram-positive cells. Blot method uses the structure of bacterial cell wall. Gram-positive bacteria

thick cell wall peptiglycan, which stains purple. Gram-negative bacteria

aa thin layer cell wall (pink spots), and they also have an outer layer containing lipids. Painting method includes four major phases: (1) a glass slide smear culture crystalline violet dye in the heart (Bunsen burner). 2) capture agent) Gram iodine) is used next. (3) Rapid de

color is treated by placing the slide (s) in alcohol or acetone. 4) Counter staining safranina done, and sometimes primary magenta is used. damage to structures. Personally PCR tests

DNA is probably more reliable for identifying species of bacteria. However, the last test is expensive, and the Gram method does not give information about which groups are most environmentally bacteria. .